Detection, quantification and characterization of Erwinia carotovora ssp. carotovora contaminating pepper seeds
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چکیده
منابع مشابه
Biological Control of Erwinia carotovora ssp. carotovora by Streptomyces Species
Ten isolates of Erwinia carotovora ssp. carotovora (Ecc) were isolated from infected potato tubers of Picasso, Sante, and Nevskiy varieties collected from different regions in Kyrgyzstan. Isolates were identified as Erwinia carotovora ssp. carotovora (Ecc) by standard bacteriological techniques and pathogenicity tests on tubers and also by PCR analyses. Tests on the pathogenicity of E. carotovo...
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Erwinia carotovora ssp. carotovora (Ecc) is a Gram-negative enterobacterium that causes soft rot in potato and other plants. The main virulence factors, secreted extracellular enzymes, degrade the plant cell wall, leading to plant tissue maceration. Extracellular enzymes, cellulases, proteases and pectinolytic enzymes are subject to complex regulatory networks including quorum sensing, two-comp...
متن کاملPolygalacturonase of Erwinia carotovora.
The polygalacturonase, clearly differentiated from polygalacturonic acid trans-eliminase, produced in the culture fluid of Erwinia carotovora was purified 56-fold by means of acetone precipitation, acetate butfer extraction, and two successive treatments by chromatography on carboxymethyl cellulose. The purified enzyme is specific for nonmethoxylated polygalacturonic acid. It shows an optimum p...
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We used a modified version of the method of Hanahan (D. Hanahan, J. Mol. Biol. 166:557-580, 1983) to transform Erwinia carotovora subsp. carotovora and E. carotovora subsp. atroseptica with the plasmids pBR322, pBR325, and pAT153. The transformation frequency ranged from 1 X 10(2) to 4 X 10(4) colonies per micrograms of plasmid DNA. The nature of these transformants was confirmed by plasmid ana...
متن کاملErwinia carotovora subsp. carotovora extracellular protease: characterization and nucleotide sequence of the gene.
The prt1 gene encoding extracellular protease from Erwinia carotovora subsp. carotovora EC14 in cosmid pCA7 was subcloned to create plasmid pSK1. The partial nucleotide sequence of the insert in pSK1 (1,878 bp) revealed a 1,041-bp open reading frame (ORF1) that correlated with protease activity in deletion mutants. ORF1 encodes a polypeptide of 347 amino acids with a calculated molecular mass o...
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ژورنال
عنوان ژورنال: Plant Pathology
سال: 2001
ISSN: 0032-0862
DOI: 10.1046/j.1365-3059.2001.00540.x